- Bee City Canada
- Birds Canada
- Bullfrog Power
- Canadian Environmental Law Association (CELA)
- Couchiching Conservancy
- Coyote Watch Canada
- Environmental Defence
- Escarpment Biosphere Conservancy
- Fatal Light Awareness Program
- Friends of the Grand River
- Friends of the Rouge Watershed
- The Fur-Bearers
- Grand River Environmental Network
- Grand Valley Trails Association
- Grand Watershed Trails Network
- Greenbelt Foundation
- Hold the Line Waterloo Region
- Journey North
- Monarch Joint Venture
- Monarch Watch
- Nature Canada
- Nature Conservancy Canada
- North American Bird Conservation Initiative (NABCI)
- North American Native Plant Society
- Oak Ridges Moraine Land Trust
- Ontario Biodiversity Council
- Ontario Headwaters
- Ontario Road Ecology Group
- Ontario Streams
- Ontario Sustainable Energy Association
- Ontario Trails Network
- RARE Charitable Research Reserve
- Reep Green Solutions
- Swim Drink Fish
- The Canadian Peregrine Foundation
- Toronto Nature Stewards
- Watersheds Canada
- Xerces Society for Invertebrate Conservation